Molecular biology is an ever-changing field, and most significant developments have happened because of changes in plasmid DNA sequencing. A plasmid, a circular and small double-stranded DNA entity ...

Sanger sequencing is the unsung workhorse of molecular biology, one whose value has long been undermined by the tedious nature of processing its data. Converting files of raw sequencing data into a ...

Genetic variations, such as mutations, recombinations, or transpositions occur naturally in cultured microorganisms and are often considered nonneutral mutations. Neutral mutations are neither ...

In 1997, Sanger, Nicklen and Coulson described a method for determining DNA nucleotide sequences. 1 Nearly five decades later, Sanger sequencing remains an entrenched technology for targeted ...